Journal: PLoS Biology
Article Title: Consolidation and maintenance of long-term memory involve dual functions of the developmental regulator Apterous in clock neurons and mushroom bodies in the Drosophila brain
doi: 10.1371/journal.pbio.3001459
Figure Lengend Snippet: (A) Five-day memory after 7-hour conditioning. ap RNAi was driven by Pdf -GAL4, c929 , and R18F07 . Box plots for a set of CI data show 10th, 25th, 75th, and 90th centiles. In the box plots, the black square in each box indicates the mean, the line in each box is drawn at the median, the white boxes indicate naive males, and the gray boxes indicate conditioned males. CI, courtship index; MI, memory index; N , sample size; U , Mann–Whitney U ; P , probability; **, P < 0.01; ***, P < 0.001; NS, not significant. (B) Five-day memory after 7-hour conditioning. ap RNAi was driven by R14F03 . *, P < 0.05; **, P < 0.01; NS, not significant. (C) l-LNv–specific expression of mCD8::GFP. c929 / R61G12 -LexA; LexAop- FLPL /UAS>STOP> mCD8 :: GFP flies were used. Brains were stained with an anti-Pdf antibody and an anti-GFP antibody. Magenta, Pdf; green, mCD8::GFP. Confocal section images at the level of Pdf neurons of the adult brain. Scale bars represent 10 μm. (D) l-LNv–specific knockdown of ap . Five-day memory after 7-hour conditioning. c929 / R61G12 -LexA; LexAop- FLPL /UAS>STOP> ap RNAi flies were used. **, P < 0.01; NS, not significant. (B and D) Box plots for a set of CI data show fifth, 25th, 75th, and 95th centiles. In the box plots, the black square in each box indicates the mean, the line in each box is drawn at the median, the white boxes indicate naive males, and the gray boxes indicate conditioned males. CI, courtship index; MI, memory index; N , sample size; U , Mann–Whitney U ; P , probability. (A, B, and D). The underlying data can be found in . Ap, Apterous; l-LNv, large ventral–lateral clock neuron; LTM, long-term memory; Pdf, pigment dispersing factor; RNAi, RNA interference.
Article Snippet: Next, the FRT-stop-FRT cassette (UAS (Addgene, 24385) and then cloned into the pUAST attB vector containing the ap RNAi fragment by using an In-Fusion HD Cloning Kit.
Techniques: MANN-WHITNEY, Expressing, Staining, Knockdown
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